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Nikon TE2000-E fluorescence microscope

eagle-i ID


Resource Type

  1. Fluorescence microscope


  1. Resource Description
    "The TE2000-E wide-field microscope is equipped for diverse imaging: * Motorized z-focusing motor enables collection of in focus images in different image planes. This utility is most often used in acquiring images for deconvolution and Stereolgy. * It has a xenon lamp for fluorescence excitation provides an excellent light source for calcium imaging with Fura-2 and UV-Vis fluorophores. * A very sensitive CoolSNAP HQ2 camera is ideal for fluorescence imaging, as it produces very little noise and allows for faster integration times. * A 5 mega pixel color camera is available for non-fluorescent samples and is ideal for histological stains. * There are 4x and 10x phase lenses available as well as high NA 20x, 40x, 60x, and 100x DIC lenses. * A computer controlled excitation filter wheel allows for fast, automated collection of images. * Filter sets are available for UV, blue, green, and red excitation and emitted light is collected with the corresponding band pass or long pass emission filters. * Filter sets are available for calcium ratio imaging, specifically fura-2 AM. * A motorized stage in conjuction with Nikon NIS Elements or Stereologer software allows for the collection of images in multiple, specified locations. * Nikon NIS Elements Advanced Research imaging software is cable of 6 dimensions (6D) of acquisition. Up to six combinations of wavelength, time, x-, y-, and/or z-positions are possible to control with this software."
  2. Contact
    Goodwin, J. Shawn, Ph.D.
  3. Contact
    Patel, Tina
  4. Manufacturer
    Nikon Corporation
  5. Model Number
  6. Website(s)
  7. Related Technique
    Fluorescent microscopy
  8. Location
    Morphology Core
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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016