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Fixation protocol for processing and paraffin embedding

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Resource Type

  1. Protocol


  1. Resource Description
    "Fixation Protocol For Processing And Parffin Embedding: • Samples submitted for processing MUST be properly trimmed (not to exceed 5 mm in thickness), and fixed in reagent of choice according to your protocol. However the most commonly used fixative is 10% Neutral Buffered Formalin (NBF). The laboratory keeps 10% NBF, sponges, and cassettes available. Samples that have been properly fixed should be transferred into 70% ethanol alcohol and taken to the laboratory for further processing. Please call the histology staff if you need guidance in these procedures. •All submitted tissue should he placed in cassettes and properly labeled with a unique ID number assigned by you. Please do not fill the cassettes to capacity with tissue. This practice will result in poor tissue processing. •Cassettes should be labeled and identified using the following system: JD08-####-tissue type. Where JD represents the first letter of the first and last name of the investigator, 00 represents the last two digits of the year, and #### is the unique ID number given by the investigator along with the tissue that is in the cassette. An example: for Jane Doe in the year 2007, 253 is the investigators unique ID number assigned by him/her and the tissue submitted is ovary. Would be: JD08-253-Ovary •Use a soft #2 pencil or Secureline Marker II/ Superfrost to label the cassettes. Do Not Use Sharpie Markers. The ink can’t with stand the chemicals used in processing. •For projects that require specially coated slides, please provide these slides at the submission of your project to the histology laboratory. Histology provides slides for routine hematoxylin and eosin (H&E) and IHC staining only. •Clearly identify all tissue containers submitted and all slides provided with investigator’s submitter’s name. •Please give one week prior notification for submissions that have a large number of samples or which will necessitate frequent and continuous submissions to the histology lab."
  2. Uses
    Tissue embedding
  3. Uses
    Histological sample preparation
  4. Used by
    Human Tissue Acquistion and Pathology Core
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016